Ultrastructure and molecular phylogeny of CW and DO groups among unicellular green algae

CW组结果见14，DO组结果见5，下同。1.对19株形成类肌碱的绿藻进行了形态和分子系统发育分析，结果表明：Chlorosarcinopsis可分为4个亚群，Dcsmotetra以近根尖基体和平行类囊体为特征；T105)应归为新属，并确定了新氯藻属（Neochlorosarcina）的有效性。重新评价Groover和Bold（1969）对类肌素成员的生理检查。2.异四芽藓属和束藻属的鞭毛不相等，彼此之间既不是单系的，也不是与其他不相等成员的亲缘关系，不相等是独立获得的。3.monticola衣单胞菌通过平行的基体和不等长的鞭毛向异四足单胞菌转移，以裸配子和前核为特征。4.从哈萨克斯坦高盐度农田土壤中分离出的一种单细胞绿藻，在提取80%的aceton时，其叶绿素A和叶绿素b的比例为2.62，并且有壁的散光zoo孢子，其鞭毛器从细胞的纵轴略微弯曲，两个鞭毛的长度相差2:1。这种生物应列入一个新属。5.毛囊菌的系统发育分析表明，毛囊菌与球褶部的小苞球菌有密切的亲缘关系。尽管它们之间存在差异，但可以推断它们的突触形态特征是存在大量盘状叶绿体。

Results of CW group are described in 1 4, and those of DO group are in 5, as next. 1.Morphological and molecular phylogenetic analyses on 19 strains of sarcinoid-forming green algae revealed that Chlorosarcinopsis are divided into 4 subgroups, that Dcsmotetra is characterized by subapical basal bodies and parallel thylakoids in pyrenoids, that Chlorosarcina stigmatica(ASIB.T105) should be classified in a new genus, and that validity of Neochlorosarcina was ascertained. Physiological examinations on sarcinoid members by Groover and Bold(1969) was re-evaluated. 2.Heterotetracystis and Fasciculochloris bearing slightly unequal flagella are neither monophyletic to one another nor closely related with other unequal members, and the unequality was independently acquired. 3.Chlamydomonas monticola should be transferred to Heterotetrachalmydomonas by parallel basal bodies and unequal flagella, and is distinguished by naked gametes and anterior nucleus. 4.A unicellular green alga isolated from a farm soil of high salinity in Kazakhstan had chlorophyll a and b at the ratio of 2.62 in extraction of 80% aceton, and walled, astigmatic zoospores whose flagellar apparatus slightly bend from longitudinal axis of cell, and lengths of two flagella differ from one another at ratio of 2:1. This organism should be included in a new genus. 5.Phylogenetic analysis of Follicularia resolved a close affinity with Bracteacoccus in the Sphaeropleales. Despite of differences between them, the presence of numerous discoid chloroplasts are inferred to be their synapomorphic feature.