Immuno-pathological studies in IgA nephropathy : The role of virus for etiological and progressive factors

伊加肾病是以伊加沉积为主的特发性肾小球肾炎。伊加肾病是世界上最常见的肾小球肾炎。然而，该病的病因仍不清楚。在伊加肾病中，肾脏症状经常在上呼吸道感染和/或胃肠道感染发作之前出现，这表明病毒是这种疾病的病因。我们以前报道过，小鼠静脉注射考克斯后，肾小球中IgG和伊加以系膜模式染色。因此，我们检测科萨基病毒和腺病毒受体（CAR），已被确定为两种病毒的共同细胞受体，表达和定位在大鼠肾小球使用嘌呤霉素氨基糖苷（PAN）肾病，发现CAR染色强度显着增加沿着肾小球毛细血管壁在PAN肾病。这些结果表明，科萨基病毒直接感染肾小球足细胞。发现反复接种科萨基B4病毒可引起抗GBM肾炎小鼠尿蛋白排泄增加和系膜增生。提示科萨基病毒B_4可加重慢性肾小球肾炎的病理状态。

IgA nephropathy is an idiopathic glomerulonephritis characterized by predominant IgA deposition. IgA nephropathy is the most common form of glomerulonephritis in the world. However, the etiology of this disease is still unclear. In IgA nephropathy renal symptoms are frequently preceded by episodes of upper respiratory tract infections and/or gastrointestinal infections, suggesting viruses as the etiology of this disease.There have been some reports on human nephritis and experimental models associated with acute viral infection, including coxsackie viruses. We previously reported that IgG and IgA were stained in a mesangial pattern in glomeruli after mice were inoculated intravenously with Cox. B4 virus monthly for 9 months although IgA deposits were not dominant.Therefore, we examined coxsackie virus and adenovirus receptor (CAR), has been identified as a common cellular receptor for both viruses, expression and localization in rat glomeruli using puromycin aminonucleoside (PAN) nephrosis and found that a significant increase in CAR staining intensity along the glomerular capillary wall in PAN nephrosis. These results suggest that coxsackie viruses directly infects in podocytes of glomeruli.And, we evaluated the efficacy of coxsackie viruses B4 for experimental models and. found that coxsackie B4 virus inoculated repeatedly into mice induces the increase of urinary protein excretion and the mesangial proliferation in anti-GBM nephritis. These findings suggest that coxsackie viruses B4 make the pathological condition of chronic glomerulonephritis worse.