Imprinted Potymer based Sensor Array Plate

1.以甲基丙烯酸和乙烯基取代的金属卟啉为功能单体，合成了（-）辛可尼定的非对映选择性分子印迹聚合物PPM（CD）。与仅使用甲基丙烯酸或金属卟啉作为功能单体的MIPs相比，PM（CD）和PP（CD）分别，PPM（CD）在使用MIPs填充柱的色谱测试中显示出更高的（-）-辛可尼定结合能力。在结合位点含有金属卟啉的MIP，PPM（CD）和PP（CD），根据（-）-辛可尼丁的结合，显示光谱变化，并且在低浓度范围内是显著的，这表明高亲和力结合位点含有卟啉残基。光谱变化与（-）-辛可尼定浓度对数的相关性呈线性关系。这些结果表明，具有高度特异性结合位点的MIP由功能单体，乙烯基取代的金属卟啉和甲基丙烯酸组装，它们协同工作，产生特异性结合。此外，基于金属卟啉的分子印迹聚合物似乎作为一种传感器选择性地响应于模板分子的结合事件.一个分子印迹聚合物库的制备在96孔微量滴定板上使用的液体处理设备，被编程为制备各种品种的辛可尼定印迹聚合物。使用酶标仪通过荧光测量筛选固定在威尔斯孔底表面上的所得聚合物的结合能力。通过与传统的FIA评估的比较，证明了基于微量滴定板的组合分子印迹的可靠性和高通量性能。

1. A diastereoselective molecubrly imprinted polymer (MIP) for (-) cinchonidine, PPM (CD), was prepared by the combined use of methacrylic acid and vinyl-substituted metallo-porphyrin as functional monomers. Compared to MIPs using only methaciylic acid or metallo-porphyrin as a functional monomer, PM (CD) and PP (CD), respectively, PPM (CD) showed higher binding ability for (-) -cinchonidine in chromatographic tests using the MIPs-packed columns. The MIPs containing a metallo-porphyrin in the binding sites, PPM (CD) and PP (CD), showed spectroscopic change according to the binding of (-) -cinchonidine and it was significant in the low concentration range, suggesting that the high affinity binding sites contain the porphyrin residue. The correlation of the spectroscopic change against log of (-) -cinchonidine concentrations showed a linear relationship. These results revealed that the MIP having highly specific binding sites was assembled by the functional monomers, vinyl-substituted metallo-porphyrin and methacrylic acid, and they cooperatively worked to yield the specific binding. In addition, the metallo-porphyrin-based MIPs appeared to act as a sensor selectively responded by binding events of the template molecule.2. A molecularly imprinted polymer library was prepared on a 96-well microtiter plate using a liquid-handing equipment that was programmed to prepare various breeds of cinchonidine-imprinted polymers. The resultant polymers immobilized on the bottom surface of the wells were screened for the binding capacity by fluorescence measurement using a microplate reader. Comparison with a conventional assessment by FIA demonstrated the reliability and the high-throughput performance of the microtiter plate-based combinatorial molecular imprinting.