Development of DNA microarray for characterization of gene network systems of fish and shellfish

对四角黄尾、牙鲆、红鲷、大菱鲆、黑斑对虾、日本袋虾和黑虎对虾进行了表达序列标记分析。我们成功地开发了黄尾虾、日本比目鱼、红鲷、黑虾和黑虎虾的互补DNA （cDNA）芯片，分别包含1,034、1,965、997,754和1,282个独特的cDNA。在脂多糖（LPS）、康纳碱A （conA）、多肽聚糖（poly IC）和肽聚糖（PG）的免疫刺激下，这些阵列载玻片被证明是有效的基因表达分析。这些载玻片也用于开发和评估针对鱼类病原体设计的疫苗。此外，对牙鲆而言，耐药菌株在感染爱德华氏菌后抗原呈递相关基因的表达量比敏感菌株上调2倍。总的来说，我们的研究表明转录组学如微阵列是研究宿主-病原体关系和可能用于药物开发的有效生物标志物工具。

We conducted expressed sequence tag analysis of yellowtail Seriola quinqueradiata, Japanese flounder Paralichthys olivaceus, red seabream, Pagrus major, kuruma shrimp Marsupenaeus japonicus and black tiger shrimp Penaeus monodon. We were able to successfully develop complementary DNA (cDNA) microarray slides for yellowtail, Japanese flounder, red seabream, kuruma shrimp and black tiger shrimp consisting of 1,034, 1,965, 997, 754, and 1,282 unique cDNAs, respectively. These array slides were used and were proven to be effective in the analysis of gene expression during immunostimulation with lipopolysaccharides (LPS), concanavaline A (conA), poly IC, and peptidoglycan (PG). These slides were also used in the development and evaluation of vaccines designed against fish pathogens. In addition, For Japanese flounder, resistant strains showed 2-fold up-regulation of antigen presentation-related genes following Edwardsiella tarda infection as compared to sensitive strains. Overall, our study shows that transcriptomics such as microarray is an effective biomarker tool in studying host-pathogen relationships and possibly for drug development.