A STUDY OF STRESS PROTEIN IN HUMAN SKELETAL MUSCLE

人类骨骼肌中应激蛋白的表达对运动和热应激的反应尚不清楚。在这项研究中，我们指出了一种安全可靠的诱导人骨骼肌应激蛋白的方法。此外，我们还研究了将我们的结果应用于临床培训领域的可能性。本研究的结果如下：(1)利用新改进的不干扰受试者日常活动和训练的针刺活检技术，建立了研究人体骨骼肌应激蛋白的方法，从而拓展了人体肌肉活检技术的研究领域。(2)众所周知，久坐状态下应激蛋白表达水平存在个体差异。我们发现，应激蛋白的表达水平与肌纤维组成有关，并且在耐力运动员中有强烈的表达。此外，瘫痪伴肌肉萎缩的患者应激蛋白表达水平较低。(3)提示局部微波热疗可以增加人骨骼肌中应激蛋白的表达。此外，我们还致力于开发微波热疗系统，为人骨骼肌中应激蛋白的诱导提供安全有效的方法。我们的方法将皮肤温度维持在40℃左右，并将肌肉温度提高到45℃，而不对肌肉造成损伤。(4)采用微波热疗系统进行肌肉预适应可以预防离心性运动所致的肌肉损伤，但这种作用可能不适用于反复进行的离心性运动。进一步的研究应该研究热应激诱导的应激蛋白的时间过程以及对运动员成绩的影响。

It was unclear that the responses of stress protein expression to the exercise and the heat-stress in human skeletal muscles. In this study, we indicated that safe and reliable modality for inducing stress proteins in human skeletal muscle. Moreover, we also investigated the possibility of applying our results to clinical training fields. The outcome of this study was shown as follows:(1) We established the approach to research stress proteins in human skeletal muscle by using newly improved needle biopsy technique which did not disturb daily activity and training of subjects Therefore, we could develop the research field with human muscle biopsy technique.(2) It is well known that there is individual variation of stress protein expression level in sedentary condition. We revealed the stress protein expression level correlated with muscle fiber composition and strongly expressed in endurance athlete. Moreover, stress protein showed less expression level in a paralysis patient with muscle atrophy.(3) We indicated that local microwave hyperthermia treatment can increase stress proteins in human skeletal muscle. Moreover, we worked on development of the microwave hyperthermia system to provide safe and effective method to induce stress proteins in human skeletal muscle. Our method maintained the skin temperature around 40℃ and increased muscle temperature until 45℃ without muscle damage.(4) The muscle preconditioning with a microwave hyperthermia system may prevent eccentric exercise-induced muscle damage, although the effect may not apply to repeated bouts of eccentric exercise.Further investigation should examine time course of stress proteins induced by heat stress, and effect on performance in athlete.