Effects of endurance training on heat shock protein in skeletal muscle of senescent animals

定期运动训练对衰老动物骨骼肌热休克蛋白（HSP）表达的影响尚不清楚。因此，本研究的目的是探讨慢性运动训练对年轻和老年大鼠骨骼肌HSP表达的影响。年轻成年（12周龄）和年老（100周龄）雌性Fisher 344大鼠被分为久坐对照组和耐力运动训练组（n=6/组）。两个训练组的大鼠都完成了跑步机运动计划（75-80%Vo2max， 60分钟/天，5天/周），持续10周。训练结束72小时后，切除比目鱼肌（SOL）和指长伸肌（EDL），采用Western blot技术检测HSP72水平。年轻和年老久坐对照动物骨骼肌HSP72基础水平无差异（p>0.05）。与年龄匹配的久坐对照组相比，运动训练导致年轻动物（SOL +22%, EDL +200%）和老年动物（SOL +15%, EDL +115%）骨骼肌中HSP72水平升高（p<0.05）。耐力训练增加了幼龄和老年动物骨骼肌中HSP72的表达。然而，随着年龄的增长，对快速骨骼肌（EDL）耐力训练的反应变得迟钝。

The effects of regular exercise training on the expression of heat shock protein (HSP) in skeletal muscle of senescent animals are unknown. Therefore, the purpose of this study was to investigate the effects of chronic exercise training on skeletal muscle HSP expression in both young and old rats. Young adult (12 weeks old) and old (100 weeks old) female Fisher 344 rats were assigned to either a sedentary control or an endurance exercise trained group (n=6/group). Rats in both training groups completed a treadmill exercise program (75-80%Vo2max, 60min/day, 5days/wk) for 10 weeks. After 72 hours on completion of the training program, the soleus (SOL) and extensor digitorum longus (EDL) muscles were excised and the levels of HSP72 were determined by Western blot technique. No differences (p>0.05) existed in the basal levels of skeletal muscle HSP72 between young and old sedentary control animals. Compared to age-matched sedentary controls, exercise training resulted in increased (p<0.05) levels of HSP72 in skeletal muscles inn both young (SOL +22%, EDL +200%) and old animals (SOL +15%, EDL +115%). Endurance training increases the expression of HSP72 in skeletal muscle in young and old animals. However, the response to endurance training in fast skeletal muscle (EDL) is blunted with an aging.