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Effects of endurance training on heat shock protein in skeletal muscle of senescent animals

耐力训练对衰老动物骨骼肌热休克蛋白的影响

基本信息

批准号：
12480011
负责人：
NAITO Hisashi
金额：
$ 5.12万
依托单位：
JUNTENDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12480011/
关键词：
international information exchange USA heat shock protein HSP72 aging exercise muscle fiber type ヒートショック蛋白質 HSP70 加齢筋トレーニング時間ラット骨格筋温浴心筋走運動持久的運動

项目摘要

The effects of regular exercise training on the expression of heat shock protein (HSP) in skeletal muscle of senescent animals are unknown. Therefore, the purpose of this study was to investigate the effects of chronic exercise training on skeletal muscle HSP expression in both young and old rats. Young adult (12 weeks old) and old (100 weeks old) female Fisher 344 rats were assigned to either a sedentary control or an endurance exercise trained group (n=6/group). Rats in both training groups completed a treadmill exercise program (75-80%Vo2max, 60min/day, 5days/wk) for 10 weeks. After 72 hours on completion of the training program, the soleus (SOL) and extensor digitorum longus (EDL) muscles were excised and the levels of HSP72 were determined by Western blot technique. No differences (p>0.05) existed in the basal levels of skeletal muscle HSP72 between young and old sedentary control animals. Compared to age-matched sedentary controls, exercise training resulted in increased (p<0.05) levels of HSP72 in skeletal muscles inn both young (SOL +22%, EDL +200%) and old animals (SOL +15%, EDL +115%). Endurance training increases the expression of HSP72 in skeletal muscle in young and old animals. However, the response to endurance training in fast skeletal muscle (EDL) is blunted with an aging.

规律运动训练对衰老动物骨骼肌热休克蛋白（HSP）表达的影响尚不清楚。因此，本研究旨在探讨慢性运动训练对青年和老年大鼠骨骼肌HSP表达的影响。将年轻成年（12周龄）和老年（100周龄）雌性Fisher 344大鼠分配到久坐对照组或耐力运动训练组（n=6/组）。两组大鼠均完成跑台运动（75-80%Vo2max，60 min/d，5d/wk），共10周。训练72 h后，取比目鱼肌（SOL）和趾长伸肌（EDL），用Western blot法检测HSP 72的表达。青年组与老年组骨骼肌HSP 72基础水平无显著性差异（p>0.05）。与年龄匹配的久坐对照组相比，运动训练导致年轻动物（SOL +22%，EDL +200%）和老年动物（SOL +15%，EDL +115%）骨骼肌中HSP 72水平增加（p<0.05）。耐力训练增加青年和老年动物骨骼肌HSP 72的表达。然而，随着年龄的增长，快速骨骼肌（EDL）对耐力训练的反应变钝。