The response of adipose tissues to oxidative stress by exercise : with special reference to the role of EC-SOD in splanchnic adioise tissue

主要研究结果如下：1.研究了急性运动对大鼠脂肪细胞β-肾上腺素能受体(β-AR)转运的影响。结果表明，运动后至少3hβ_2-AR密度升高的机制可能与参与β_2-AR转运的蛋白质之间的协同作用有关，其中受体-激动剂相互作用和泛素-蛋白酶体途径都起着关键作用。β_2-AR蛋白表达下降可能是由于在翻译水平上发生了变化。研究了运动训练对大鼠脂肪细胞氧化应激的影响。因此，内脏脂肪组织中的硫代巴比妥酸反应物质(TBARS)水平显著降低，但外周脂肪组织中的水平并未降低。同时，细胞外超氧化物歧化酶(EC-sod)水平似乎只在内脏脂肪…中升高。更多的硒组织。考虑到训练后内脏脂肪组织中单核细胞趋化蛋白-1(MCP-1)水平的降低，研究结果表明，运动训练降低了内脏脂肪组织中的氧化应激，从而改善了与生活方式相关的疾病。研究了从荔枝中提取的低分子多酚低聚醇(日本札幌氨基UP化学有限公司)对小鼠脂肪细胞的抗氧化作用。尽管给予高脂饮食(HFD)的小鼠的内脏脂肪组织质量和TBARS水平显著高于对照组，但寡糖醇肯定地抑制了HFD诱导的这种增加。肾衰小鼠内脏脂肪组织中肿瘤坏死因子-α和单核细胞趋化蛋白-1基因的表达也显著高于对照组和肾衰低聚醇组。此外，EC-SOD基因的表达仅在HFD+低聚醇小鼠的内脏脂肪组织中增强。这些结果表明低聚醇对氧化应激有良好的作用。较少

The main findings obtained during 2005-2006 are as follows:1. The effect of acute exercise on β-adrenergic receptor (β-AR) trafficking was investigated in rat adipocytes. As the result, the mechanism underlying the increased density of β_2-AR up to at least 3 h after exercise might involve alterations in a multistep event involving the coordinate interaction among proteins mediating β_2-AR trafficking, in which both the receptor-agonist interactions and ubiquitin-proteasome pathway have a key role. However, the decreased protein expression of β_2-AR at 24 h might be due to some change occurring at the translational levels.2. The effect of exercise training on oxidative stress was investigated in rat adipocytes. In consequence, the level of thiobarbituric acid-reactive substances (TBARS) was significantly decreased in splanchnic adipose tissue, but not in peripheral adipose tissue. Meanwhile, extracellular superoxide dismutase (EC-SOD) level appeared to increase only in splanchnic adipo … More se tissue. Considering reduced level of monocyte chemoattractant protein-1 (MCP-1) in splanchnic adipose tissue after training, the findings obtained suggest that physical training reduces oxidative stress in splanchnic adipose tissue, resulting in improvement in lifestyle-related disease.3. The antioxidative effect of new lychee-derived polyphenol Oligonol converted into a low-molecular form (Amino Up Chemical Co., Ltd., Sapporo) was investigated in mouse adipocytes. Although the levels of splanchnic adipose tissue mass and TBARS in mice given a high fat diet (HFD) were significantly higher than those in control mice, Oligonol definitely inhibited such HFD-induced increases. Expression of TNF-α and MCP-1 genes in splanchnic adipose tissue of HFD mice was also significantly higher than that in control and HFD + Oligonol mice. Moreover, the expression of EC-SOD gene was enhanced only in splanchnic adipose tissue of HFD + Oligonol mice. These results suggest that Oligonol has favorable effects on oxidative stress. Less