Dermal wound aging by molecular biological detections of growth factors

皮肤创伤年龄为法医病理学家提供了宝贵的信息，有助于重建犯罪现场和确定死因。在这项研究中，使用免疫组织化学方法、实时定量聚合酶链式反应和原位杂交技术分析了小鼠皮肤中细胞因子的表达。基于17种细胞因子的量化，还进行了人类皮肤伤口的伤口年龄估计。创面愈合早期为IL-5、IL-10、IL-12p70、IL-13、IL-17、GM-CSF、干扰素γ、肿瘤坏死因子α，中期为IL-6、单核细胞趋化蛋白1，中晚期为IL-1β、IL-2、IL-4、IL-8、G-CSF、MIP-1β。此外，还进行了任何法医机构均可获得的IL-1β、IL-4、IL-6、IL-8、IL-10、粒细胞集落刺激因子、粒-巨噬细胞集落刺激因子、干扰素γ、单核细胞趋化蛋白1、肿瘤坏死因子α的外包检测。许多因素已被提出作为皮肤创伤年龄估计的标志，但本研究中为外包检查选择的一组细胞因子将在日常法医实践中有用。

Dermal wound age provides forensic pathologists with valuable information for the reconstruction of crime scenes and determination of the cause of death. In this study, cytokine expressions in murine skins were analyzed using immunohistochemical methods, real-time quantitative PCR, and in situ hybridization techniques. Wound age estimation for human dermal wounds was also performed based on quantification of 17 cytokines. The potential parameters would be IL 5, IL 10, IL 12 p 70, IL 13, IL 17, GM-CSF, IFN γ, TNF α in the early phase of dermal wound healing, IL 6, MCP 1 in the middle phase, and IL 1 β, IL 2, IL 4, IL 8, G-CSF, and MIP 1β in the middle and late phases. In addition, an outsourced examination of IL 1 β, IL 4, IL 6, IL 8, IL 10, G-CSF, GM-CSF, IFN γ, MCP1, TNFα, which could be available to any forensic institute, was performed for confirmative purposes. Many factors have been proposed as markers for dermal wound age estimation, but the set of cytokines selected for the outsourced examination in the present study wound be useful in daily forensic practice.