Inducible mechanism and role of novel aggrecanase in early stage of arthritis and its therapeutic application

1侵袭的诱导机制取新生大鼠膝关节，分离软骨细胞。施加机械应力，检测血管紧张素转换酶的表达。ADAMTS5不受机械应力的影响，而ADAMTS1、4和ADAMTS9的mRNAs随着机械应力的增加而增加。2 ADAMTS9在软骨中的分布我们建立了Wistar大鼠骨关节炎模型。当软骨破坏不是很严重时，ADAMTS9已经被诱导表达。然后检测ADAMTS9在发育膝关节中的表达。分别于出生后0、7、14周取ICR小鼠膝关节。ADAMTS9在成熟软骨细胞层(部分)和肥大带有表达。这些实验表明，ADAMTS9与膝关节软骨细胞的成熟有关。

1 Inducible mechanism for aggrecanaseChondrocytes were isolated from new born rat knee joint. Mechanical stress was performed and the expressions of aggrecanases were examined. ADAMTS5 was not increased by mechanical stress, while mRNAs of ADAMTS1,4, and ADAMTS9 were increased by mechanical stress. Then, the2 Distribution of ADAMTS9 in cartilageWe produced osteoarthritis model in Wister rats. When the cartilage destruction was not so severe, the expression of ADAMTS9 was already induced. Then we examined the expression of ADAMTS9 in developmental knee joint. The knee joint was taken at 0, 7, and 14 weeks after birth in ICR mice, respectively. The expression of ADAMTS9 was observed in mature chondrocyte layer (partially) as well as hypertrophic zone. From these experiments, it is suggested that ADAMTS9 is related to the maturation of chondrocytes in knee joint.