Antitumor effect of acridine orange under ultrasonic irradiation in vitro

背景：声动力协同抗肿瘤治疗是一种很有前途的肿瘤治疗新方法。材料与方法：为研究吖啶橙（AO）存在下超声（US）的抗肿瘤作用，采用1.0 μg/ml AO溶液作为声动力化合物体外抗180肉瘤细胞。结果：2.0 W/cm^2 US照射60秒后，有AO的肿瘤细胞存活率明显低于无AO的肿瘤细胞存活率（p<0.001）。在ao治疗组，肿瘤细胞多呈碎片化。当d -甘露醇或l -组氨酸与AO同时使用时，肿瘤细胞的辐照存活率明显高于单独使用AO时。结论：AO在US照射下具有良好的抗肿瘤活性，单线态氧自由基和羟基自由基的生成参与了肿瘤细胞损伤的过程。

Background: Sonodynamic synergistic antitumor therapy is a promising new methodology for cancer treatment.Materials and Methods: To determine the antitumor effects of ultrasound (US) in the presence of acridine orange (AO), 1.0 μg/ml solution of AO was tested as a sonodynamic compound against sarcoma 180 cells in vitro.Results: After US irradiation at 2.0 W/cm^2 for 60 sec, the survival rate of tumor cells in the presence of AO was significantly lower than in its absence (p<0.001). In the AO-treated group, the tumor cells were mostly fragmented. When D-mannitol or L-histidine was used along with the AO, the survival rate of tumor cells after irradiation was significantly higher than that when AO alone was applied.Conclusion: AO could exhibit useful antitumor activity under US irradiation, and the generation of singlet oxygen and hydroxyl radicals is involved in the processes of tumor cell damage.