Construction of yogurt with strong exclusion ability of harmful bacteria by probiotic blood type lactic acid bacteria

通过识别ABO血型抗原筛选粘附人结肠粘蛋白的益生菌乳酸菌(LAB)的一种新的评价系统。利用Biacore生物传感器从237株益生菌中筛选出对肠道黏膜上表达的人A型血型抗原有很强粘附性的实验室菌株。一种类似的方法被用来选择也识别人类肠道中相同抗原的有害细菌。葡萄球菌。SSP。和大肠埃希菌均检出有害菌。用涂有A型抗原的微孔板与有害细菌进行竞争性黏附实验，当它们与A型实验室联合加入时，细菌显著减少。我们的数据表明，血型实验室菌株可能具有竞争性地抑制病原体感染，这一策略可能有助于开发改进的未来益生菌食品，以保护人类健康。

A new evaluation system to select probiotic lactic acid bacteria (LAB) with adherence to human colonic mucin (HCM) by recognizing ABO-blood type antigens. LAB strains showing strong adhesion to human blood type-A antigen expressed on the intestinal mucosa were selected from 237 probiotic strains using the biosensor BIACORE. A similar method was used to select harmful bacteria that also recognizing the same antigen in the human intestine. Staphyrococcus. ssp. and E. coli were detected harmful bacteria. Using a competitive adhesion experiment with a micro plate coated with the type-A antigen and the harmful bacteria, the bacteria was significantly decreased when they were added in combination with the type-A LAB. Our data suggested the blood type LAB strains may competitively inhibit pathogenic infections where this strategy may be useful in the development of improved future probiotic foods to protect human health.