Elucidation of the molecular mechanism underlying the enhancement of somatic-cell reprogramming by a histone deacetylase inhibitor, and its applications

体细胞核移植（SCNT）效率极低，自第一次克隆绵羊“多莉”以来，成功率不到5个百分点。该基金的代表研究人员发现，用组蛋白去乙酰化酶抑制剂（HDACi）曲古霉素A （TSA）处理克隆胚胎，可将克隆小鼠的繁殖率显著提高6倍以上。在这项资助的支持下，研究人员揭示了HDAC在胚胎发育中的重要性以及HDAC治疗改善SCNT的机制，并首次实现了“不可克隆”小鼠品系的进一步克隆。

Somatic-cell nuclear transfer (SCNT) has been extremely inefficient with rates less than 5 percentage points since the first clone, "Dolly" the sheep. The representative researcher for this grant found that treatment of cloned embryos with trichostatin A (TSA), a histone deacetylase inhibitor (HDACi), dramatically increase the production rate of cloned mice more than six-times. Supported by this grant, the importance of HDAC in embryonic development and a mechanism underlying improvement of SCNT by HDACi treatment were revealed and, further cloning of "unclonable" mouse strains were achieved for the first time.