Generation of MicroAntibody by directed evolution

在本研究中，我们开发了一种结合蛋白质结构设计和定向进化的方法来缩小抗体的尺寸。我们构建了一个具有非免疫球蛋白结构域的选择性结合肽库，用于开发分子探针和分子靶向药物。螺旋-环-螺旋肽是一种特异性结合靶分子的多肽，被称为“微抗体”。我们已经构建了一个微抗体库，以产生人IgG-Fc特异性肽和Aurora激酶特异性肽。并对微抗体的免疫原性和稳定性进行了研究

In this study, we have developed a method to downsize the antibody by the combination of structure-design of proteins and directed evolution. We have constructed a library of alternative-binding peptide with non-immunoglobulin domain to develop molecular probe and molecular targeting drug. The helix-loop-helix peptide, which binds to specifically target molecules, is named as "MicroAntibody". We have constructed a library of MicroAntibody to generate human IgG-Fc-specific peptides and Aurora kinase-specific peptides. We also studied immunogenicity and stability of MicroAntibody