Biochemical and genetic analysis of initiation of eukaryotic genomic DNA replication

在真核生物中，许多起始因子在染色质上组装和分解，其中一个因子Mcm10被认为在复合体的形成中起着重要作用。我仔细评估了Mcm10如何参与DNA复制。MCM10的温敏等位基因mcm10-1在不允许的条件下，从G1区释放后，显著降低了DNA的复制。生化分析表明，芽殖酵母Mcm10形状细长，形成单体，能直接与某些DNA复制起始因子结合。

In eukaryotes, many initiation factors are assembled and disassembled on chromatin, and one of the factor, Mcm10, was thought to be involved in important role for the complex formation. I carefully assessed how Mcm10 participates in DNA replication. A thermosensitive allele of MCM10, mcm10-1 significantly reduced DNA replication at the nonpermissive condition after release from G1 block. Biochemical analysis revealed that budding yeast Mcm10 is a long and narrow shape and form a monomer, and it can bind to some DNA replication initiation factors directly.