Prediction of radiosensitivity using phosphorylation of histone H2AX in human tumor cell lines

为了开发一种新的放射敏感性预测方法，我们使用7种不同的人类细胞系测试了细胞存活与组蛋白H2AX（γH2AX）磷酸化表达之间的关系。γ - h2ax聚焦频率与辐照后存活分数相关性不强。将DNA含量归一化γ - h2ax聚焦频率，并引入凋亡频率进行分析，除1株细胞株外，其余6株细胞株的细胞存活率与凋亡频率和γ - h2ax聚焦频率的结合有较好的相关性。

To develop a new predictive assay for radiosensitivity, we tested the relationship between cell survival and expression of phosphorylation of histone H2AX(γH2AX) foci using 7 different human cell lines. There was not a good correlation between the γH2AX foci frequency and surviving fraction after irradiation. When we normalized theγH2AX foci frequency by DNA content, and introduced the frequency of apoptosis to the analysis, there was a good correlation between the cell survival and the combined frequency of apoptosis and γH2AX foci in 6 cell lines except for one.