Molecular mechanism of neurite outgrowth regulated by PLCdelta3

磷脂酶Cδ3(Pholipase Cδ3，PLCDAG 3)是一种在神经元组织中高度表达的蛋白，它能降解磷脂酰肌醇4，5-二磷酸(PI(4，5)P_2)，产生两个第二信使，即IP_3和DAG。电穿孔基因敲除E14胚胎大脑皮层内的PLCδ3，可抑制发育中的大脑皮层神经元的迁移。此外，我们还证明，当PLCδ3在分化过程中被瞬时击倒时，脑神经元、小脑颗粒细胞或Neuro2a细胞的神经元突起受到抑制。

Phospholipase Cδ3(PLCδ3), which is highly expressed in neuronal tissues, hydrolyzes phosphatidylinositol 4, 5-bisphosphate(PI(4, 5) P_2) and generates two second messengers, i. e. IP_3 and DAG. PLCδ3 knockdown in cerebral cortex of E14 embryo by in utero electroporation caused the migratory inhibition of cortical neurons in developing cerebral cortical plate. Furthermore, we demonstrated that neuronal outgrowth was inhibited in cerebral neurons, cerebellar granule cells, or Neuro2a cells when PLCδ3 was transiently knocked down during differentiation.