肺腺癌の転移成立を防ぐための研究

经EGFR酪氨酸激酶抑制剂（TKIs）处理后，悬浮培养系统中EGFR突变肺腺癌细胞发生大量凋亡。另一方面，单层培养系统中的EGFR突变细胞比悬浮肿瘤细胞更能抵抗EGFR tks诱导的凋亡，尽管在两种培养条件下，EGFR突变细胞中表达的EGFR磷酸化都被完全抑制。此外，EGFR突变型肺腺癌患者转移阳性淋巴结中的瘤内浮动肿瘤细胞表达突变特异性EGFR的方式与细胞外基质黏附肿瘤细胞相同。这些发现表明，漂浮在血管中的肿瘤细胞可能对EGFR TKIs高度敏感。

EGFR-mutant lung adenocarcinoma cells in suspension culture system undergo massive apoptosis by treatment with EGFR tyrosine kinase inhibitors(TKIs). On the other hand, EGFR-mutant cells in monolayer culture system are much more resistant to EGFR TIKs-induced apoptosis than suspended tumor cells, although the phosphorylation of EGFR expressed in EGFR-mutant cells is completely suppressed in either culture condition. Also intrasinus floating tumor cells in metastasis-positive lymph nodes from EGFR-mutant lung adenocarcinoma patients expressed mutant specific EGFR in the same way as extracellular matrix adhesive tumor cells. These findings suggest that the tumor cells floating in vessels would be highly susceptible to EGFR TKIs.