Interaction Human hepato-cell between Hepatocellular carcinoma specific HBV mutation

我们曾报道HBx 94、HBx131和HBx130氨基酸突变与肝细胞癌患者感染乙肝病毒有关。我们制备了野生型3HBx转基因小鼠(野生型TG小鼠(2株)、WM型转基因小鼠(2株)和MM TG小鼠(3株))。野生型转基因小鼠为野生型HBx转基因小鼠。WmTg小鼠为HBx，HBx131和HBx130氨基酸突变表达转基因小鼠。MM TG小鼠为HBx转基因小鼠，HBx 94、HBx131和HBx130氨基酸突变表达转基因小鼠。在野生转基因小鼠中，肝细胞癌的发生依赖于HBx的表达数量。MM TG小鼠HBx的表达相对弱于野生型和WM TG小鼠，但MM TG小鼠发生了肝细胞癌。基因1和细胞周期蛋白B1mRNA在3HBx转基因小鼠中的表达高于B6j小鼠。但3只表达HBx的转基因小鼠在上述表达上无明显差异。

We previously reported that HBx 94, HBx131 and HBx130 amino acid mutations associate with HCC patients infected with hepatitis B virus. We made wild 3 HBx expressing transgenic mouse(wild TG mouse(2 strain), WM TG mouse(2 strain) and MM TG mouse(3 strain)). Wild TG mouse was wild HBx expressing transgenic mouse. WM TG mouse was HBx with HBx131 and HBx130 amino acid mutations expressing transgenic mouse. MM TG mouse was HBx with HBx 94, HBx131 and HBx130 amino acid mutations expressing transgenic mouse. In wild TG mice, hepatocellular carcinogenesis is dependent on quantity of HBx expression. MM TG mice are relatively smaller expression of HBX than wild TG mouse and WM TG mouse, but HCC developed in MM TG mouse. Gene1(for so it will be convenient to speak of it) and cyclin B1 mRNA were expressed more in 3 HBx expressing transgenic mouse than in B6j mouse. But there was not difference among 3 HBx expressing transgenic mice on these expression.