Development of reverse-genetics methods for Daphnids

水蚤（Daphnia magna）是一种小型甲壳动物，在淡水中无处不在。由于水蚤栖息在各种各样的水生环境中，它们是理解对环境变化和进化的反应和适应的一个很好的模型。因此，它们已被用作生态毒理学的标准生物之一。虽然水蚤在生态毒理学中具有重要意义，但了解水蚤对环境变化反应的工具非常有限。我们研究了自然雄性产生的条件，并改进了将外源DNA导入水蚤的方法。这些基本信息将为转基因蚤类的建立提供依据。

The water flea Daphnia magna is a small crustacean which is an ubiquitous inhabitant in fresh water. As daphnids inhabit a diverse array of aquatic environments, they are a good model for understanding response and adaptation to environmental changes and evolution. Therefore, they have been used one of standard organisms in ecotoxicology. While daphnids have significant importance in ecotoxicology, tools to understand response to environmental changes in daphnia is very limited. We investigate a condition for natural male production and improve introduction of foreign DNA into daphnids. These basic information will be available for establishment of transgenic daphnids.