Analysis of the mechanisms of phosphorus homeostasis in major organ using high-phosphorus diet administrated rat model and its application

雄性Wistar大鼠10只，随机分为两组，分别饲喂含磷0.3%(C)和1.2%(HP)的饲料24天。使用这些动物的肾脏、肝脏和白色脂肪组织(WAT)进行DNA微阵列分析。等级聚类分析表明，两组的基因表达谱彼此不同。在两组差异表达的基因中，Hp大鼠肾脏IIb型钠依赖磷酸转运蛋白(Napi-IIb)显著上调。在肝脏方面，观察到HP饮食可上调脂肪酸β-氧化，下调氨基酸分解代谢过程。在HP大鼠的WAT中也观察到与脂肪酸β-氧化相关的基因上调。因此，我们的结果表明，高磷饮食不仅诱导了磷酸盐稳态的调节，而且还改变了各种代谢途径。

Ten male Wistar rats were divided into two groups and fed a diet containing 0.3% (C) or 1.2% (HP) phosphorous for 24 days. DNA microarray analyses were performed using kidney, liver and white adipose tissue (WAT) of these animals. A hierarchical clustering analysis revealed that gene expression profiles of the two groups are different from each other. Among the genes that were differentially expressed between the two groups, there was significant upregulation of type IIb sodium-dependent phosphate transporter (NaPi-IIb) in the kidney of HP rats. As for the liver, upregulation of fatty acid beta-oxidation and downregulation of amino acid catabolic process by HP diet administration were observed. Upregulation of genes related to fatty acid beta-oxidation was also observed in the WAT of HP rat. Thus, our results indicate that administration of high phosphorus diet induced not only regulation of phosphate homeostasis but also changes in various metabolic pathways.