Structural and biological studies on metabolism of active oxygen species in medicinal plants

过氧化氢发生在植物细胞中，已知会给植物体带来严重的压力。许多植物在体内建立了解毒过氧化氢的系统，而过氧化物酶催化这种解毒。为了准确地了解这些解毒反应的机制，我试图用x射线晶体学证明吗啡过氧化物酶和黄芩素过氧化物酶的结构。结果，我在大肠杆菌中表达了大量的重组蛋白，而它们是不溶的，并且失去了过氧化物酶活性。因此，当我重新折叠不溶性和无活性的蛋白质时，得到了活性形式的两种过氧化物酶。此外，我们还结晶了吗啡过氧化物酶。

Hydrogen peroxide, which occurs in plant cells, is known to give serious stress to plant bodies. Many plants construct the systems to detoxify hydrogen peroxide in their bodies, and peroxidases catalyze this detoxification. To precisely understand the mechanism of these detoxifying reactions, I attempted to demonstrate the structures of morphine peroxidase and baicalein peroxidase by X-ray crystallography. As results, I expressed large amounts of recombinant protein in E. coli, whereas they were insoluble and lost the peroxidase activity. Therefore, when I refolded the insoluble and inactive protein, and obtained both peroxidases in the active forms. In addition, we crystallized morphine peroxidase.