Effect of bleeding rate on renal dysfunction following hemorrhagic shock in rat

以非出血组为研究对象，将出血量达到全身总血容量25%的三组大鼠分别出血5、20、60分钟，另一组大鼠出血60分钟，并给予细胞因子抑制剂处理。MBP的回收；血浆去甲肾上腺素、肾上腺素和醛固酮的分泌；促炎细胞因子的表达，如TNF-a和IL-1b，随着出血率的增加而恶化，这是由于失血性休克的自我调节血流动力学反应减弱。血尿素氮、血肌酐浓度、动脉血气分析及肾脏组织损伤程度与出血率降低程度有良好的相关性。出血率通过抑制血流动力学自身调节反应和促炎细胞因子的表达而成为出血性肾功能障碍的关键因素之一。

The effect of bleeding speed on renal dysfunction was studied using non-hemorrhaging group, three groups of rats who were bled up to 25% of total body blood volume for 5, 20 and 60 minutes, and the other one was bled for 60 minutes treated with cytokine inhibitors. The recovery of MBP; secretion of plasma norepinephrine, epinephrine, and aldosterone; the expression of proinflammatory cytokines, such as TNF-a and IL-1b, deteriorated as the bleeding rate increased due to a blunting autoregulatory hemodynamic responses to hemorrhagic shock. The severity of renal dysfunction, manifested by blood urea nitrogen, serum creatinine concentration, arterial blood gas analysis and histological damage in the kidney, were well correlated with the degree of decrease in bleeding rate.The bleeding rate was one of the key factors on hemorrhagic renal dysfunction through the suppression of hemodynamic autoregulatory responses and the expression of proinflammatory cytokines.