Pharmacokinetic analysis of site-directed and light-sensitive liposome

本研究以光敏物质维替泊芬为原料，制备了定点光敏脂质体（SDLSL）。532nm和698nm的激光辐照能有效地破坏SDLSL。我们还将抗癌药物顺铂（CDDP）包裹到SDLSL中，以进一步研究激光照射对CDDP释放的影响。结果，我们观察到抗癌剂的释放。我们在体外和体内验证了包封SDLSL的CDDP的肿瘤抑制功效。以结肠癌细胞株Colon26为研究对象，光照射组在体外和体内均能明显抑制肿瘤细胞的生长。从这些结果，SDLSL可以是一个非常有效的定点药物输送系统在体外和体内。使用SDLSL作为药物递送的媒介物，应当实现对位点定向组织的实质性药理作用和最小副作用。

The site-directed and light-sensitive liposome (SDLSL) was prepared using a light-sensitive substance verteporfin in this study. SDLSL could be effectively destroyed by laser irradiation of 532nm and 698nm. We also encapsulated the cisplatin (CDDP), an anticancer agent, into SDLSL in order to investigate further to check the release of CDDP by laser irradiation. As a result, we observed the release of anticancer agent. We validated tumor-suppressive efficacy of the CDDP encapsulating SDLSL in vitro and in vivo. Using colon 26 cell line and CDDP encapsulating SDLSL, strong cancer cell growth inhibitory effect was obtained with light irradiation group in vitro and in vivo, respectively. From these results, SDLSL can be a highly efficacious site-directed drug delivery system in vitro and in vivo. Using SDLSL as a vehicle for drug delivery, substantial pharmacologic effects with minimum side effects into site-directed tissues should be achieved.