Induction of oral dryness and endocannabinoid

本研究旨在阐明内源性大麻素与口渴之间的关系。实验选用雄性Wistar大鼠。首先，用膜片钳技术研究了内源性大麻素对大脑口渴中枢穹隆下器(SFO)神经元IPSCs的影响。CB1受体激动剂Win 55,221-2(Win)显著降低IPSCs的频率，但不降低其幅度。CB1拮抗剂的作用消失。其次，用多电极阵列(MEA)方法研究了内源性大麻素对SFO神经活动的影响。应用内源性大麻素ANANANDAME和WIN只影响少量神经元的单位活动。重症监护室。注射Win对水和盐的摄入量没有影响。这些表明，虽然内源性大麻素影响SFO中GABA的释放，但在正常情况下，如果存在的话，它们在诱导口渴感觉方面并不起任何重要作用。

This study was to clarify a relationship between endocannabinoid and thirst sensation. Male Wistar rats were used in the experiment. First, the effects of endocannabinoids on IPSCs of neurons of the subfornical organ (SFO), which is a thirst center of the brain, recorded from brain slice preparations were investigated with patch clamp method. The CB1 receptor agonist WIN 55,221-2 (WIN) decreased significantly the frequency but not the amplitude of IPSCs. The effect was disappeared with the CB1 antagonist. Next, the endocannabinoids on neural activity of the SFO were investigated with the multi-electrode array (MEA) method. Application of the endocannabinoid anandamide and WIN affected unit activities in only the small number of neurons. The i.c.v. injection of WIN had no effects on water and salt intakes. These suggest that while endocannabinoids affect GABA release in the SFO, they do not play any important role in induction of thirst sensation, if present, in the normal condition.