Optically regulating the synaptic plasticity at the level of single synaptic spine.

作为建立利用雷帕霉素诱导的蛋白二聚化模式操纵突触传递的技术方法的初步步骤，构建了两个质粒文库；基于psd -95的构造库和基于Rho GTPase家族的库。这些文库将有助于发展基于雷帕霉素的方法来研究神经元细胞的突触可塑性。为了评估雷帕霉素诱导的神经元内蛋白二聚化，还建立了监测细胞类型中可溶性蛋白扩散动力学的方法。

As a preliminary step toward the establishment of technical methods to manipulate the synaptic transmission by rapamycin-induced protein dimerization paradigm, two plasmid libraries were constructed; PSD-95-based construct library and Rho GTPase family-based library. These libraries will contribute to the development of rapamycin-based methods to study synaptic plasticity in neuronal cells. To evaluate the rapamycin-induced protein dimerization within neurons, methods to monitor the diffusion kinetics of soluble proteins in the cell type were also established.