Development of oral immunotherapy for allergies using TGF-betacontaining egg white derived from transgenic avian

本研究中，我们试图通过基因改造鸡，使其产生从杉木花粉和抗过敏细胞因子TGF-ss中提取的人类T细胞表位，从而通过诱导口服耐受来治疗花粉过敏。含t细胞表位的蛋清可显著降低过敏小鼠的喷嚏次数和过敏原特异性IgE水平。利用输卵管特异性表达系统制备了含TGF-ssgene的转基因鸡。转基因鸡模型将扩大治疗性蛋白在鸡蛋中的使用，可作为基于口服耐受性的过敏免疫治疗的食用药物。

Here, we attempted to generate genetically manipulated chickens producing human T -cell epitopes derived from Japanese cedar pollen and anti-allergic cytokine, TGF-ss in order to develop a treatment fro pollen allergy by inducing oral tolerance. The number of sneezing and the allergen-specific IgE level were significantly decreased in allergic mice fed with T-cell epitope-containing egg white. GM chickens harboring TGF-ssgene were generated using oviduct-specific expression system. GM chicken model will extend the usage of therapeutic proteins in eggs, which may serve as edible pharmaceuticals for allergy immunotherapy based on oral tolerance.