Elucidation of genetic factors for Parkinson disease employing next-generation sequencer

我们使用下一代测序仪对汇集的DNA进行重测序，以鉴定与罕见变异相关的疾病。从8个混合DNA样本中制备了16组混合DNA。每组汇集的dna进行聚合酶链反应以扩增目标基因，汇集到一个管中并进行条形码索引，然后使用illumina GAIIx进行广泛的序列分析。通过优化数据处理以解决整数规划问题，我们能够从64个样本中提取新的变体。

We applied resequencing of pooled DNA using a next-generation sequencer to identification of disease associations with rare variants. Sixteen sets of pooled DNAs from eight pooled DNA samples were prepared. Each set of pooled DNAs was subjected to polymerase chain reaction to amplify the target gene, pooled into one tube with barcode indexing, and then subjected to extensive sequence analysis using theIllumina GAIIx. With the optimization of data processing to solve integer programming problems, we were able to extract novel variants from 64 samples.