Development of a novel technique to monitor gene expression profiles of pathogenic bacteria during infection process

对病原体对宿主环境的基因表达的分析为理解潜在事件提供了线索，从而确定了传染病。在这项研究中，我们建立了一种新的方法，称为IVET-IP(体内表达标签免疫沉淀系统)，使我们能够识别在宿主环境中表达的基因。利用该方法，我们测定了支气管败血波氏杆菌在大鼠感染过程中的基因表达。因此，我们发现细菌毒力基因的体内表达模式与体外表达模式有很大的不同。此外，至少有289个支气管炎波氏杆菌基因位点在大鼠体内表达，是体外表达的2倍。

Analyses for gene expression of pathogens in response to the host environment provide clues to understanding the underlying events to establish the infectious diseases. In this study, we established a novel method named IVET-IP (in vivo expression tag immunoprecipitation system) to enable us to identify genes expressed in the host environment. By using this method, we determined genes expressed in Bordetella bronchiseptica in rats during infection process. As a result, we found that the in vivo expression pattern of bacterial virulence genes were quite different from that in vitro. In addition, at least 289 gene loci of Bordetella bronchiseptica were found to be expressed >2-fold in rats compared to in vitro.