Genetic test as an exposure assessment of hazardous chemical

DNA甲基化调查了作为危险化学品暴露指数的效用。首先，我们检测了有害化学物质对基因组DNA的DNA甲基化，长期给予Arat重金属镉，并检测了大鼠肝脏基因组DNA ID序列的DNA甲基化。以甲基化敏感性限制性内切酶为模板，采用实时荧光定量聚合酶链式反应方法对其进行分析。结果，CD的DNA甲基化比率变高。接下来，我们检测了从受试者(女性)血液中提取的基因组DNA的Alu序列的DNA甲基化。我们用DNA甲基化特异的引物对其进行实时定量聚合酶链式反应分析。在重金属(Cd、Pb、Hg)及其相关性中没有发现DNA甲基化的比例，但Alu序列的DNA甲基化反映了骨密度和相关性，因此被认为是骨性指标之一。

DNA Methylation investigated utility as exposure indices of hazardous chemicals. We did a target gene with interspersed repeated sequence to examine global DNA methylation.Firstly we examine DNA methylation of genomic DNA by hazardous chemicals, we gave arat heavy metal cadmium for a long term and examined DNA methylation of ID sequence of genomic DNA in rat liver . Using methylation sensibility restriction endonuclease, we analyzed it by real-time PCR method. As a result, a ratio of DNA methylation became high by Cd. Next we examined DNA methylation of Alu sequence of the genomic DNA which extracted it from blood of the subject (female). We used the primer which DNA methylation was specific for and analyzed it by real-time PCR method. The ratio of DNA methylation was not found in the heavy metal (Cd,Pb,Hg) and the correlation, but it is thought that it is it in one of the osseous bone index as for the DNA methylation of Alu sequence because it revealed bone density and correlation.