New Transgenic Mouse System to Analyze Functional Mechanisms of Transcription Factors

转录因子与非dna结合辅因子、染色质重塑酶等多种伙伴蛋白形成多蛋白复合物，调控多种靶基因的表达模式。在这项研究中，我们产生了表达含有串联亲和纯化（TAP）标签的转录因子的转基因小鼠系。这些转基因小鼠以诱导的方式组织依赖地表达tap标签蛋白，并适用于各种实验，如转录辅助因子的质谱分析以及靶基因的chip测序。这些研究将有助于解剖体内转录调控的分子机制。

Transcription factors form multi-protein complexes with various partner proteins including non-DNA binding co-factors and chromatin remodeling enzymes, and regulate expression patterns of numerous target genes. In this study, we generated transgenic mouse lines that express a transcription factor containing Tandem Affinity Purification (TAP)-tag. These transgenic mice express TAP-tag proteins tissue-dependently in an inducible manner, and are applicable to various experiments such as mass spec analyses of transcriptional co-factors as well as ChIP-sequening of target genes. Such studies will help dissection of molecular mechanisms of in vivo transcriptional regulation.