Study of a novel insulin signaling network mediated by GAPDH in H9c2 cardiac cell line

在大鼠心肌细胞衍生细胞系H9c2中，研究表明胰岛素治疗可诱导糖酵解酶之一GAPDH的磷酸化。在本研究中，在H9c2细胞系中，胰岛素处理诱导GAPDH硝化及其与肌动蛋白的关联在GAPDH对胰岛素刺激的磷酸化反应之前发生。此外，在用胰岛素处理细胞系后，观察到细胞核中GAPDH蛋白的积累。我们还证明了GAPDH介导的胰岛素信号在2型糖尿病大鼠心脏中受损的可能性。这些结果提示GAPDH可能在心肌胰岛素信号转导中起关键作用。

In the rat cardiomyocyte-derived cell line H9c2 it has been shown that phosphorylation of GAPDH,one of the glycolytic enzymes, was induced by insulin treatment. In this study, GAPDH nitration and its association with actin were both induced by insulin treatment before the GAPDH phosphorylationresponse to insulin stimulation in the H9c2 cell line. Moreover, after treatment of the cell line with insulin, accumulation of GAPDH protein in the nucleus was observed. We also demonstrated the possibility that insulin signaling mediated by GAPDH was impaired in the heart of type 2 diabetic rat. These results suggest that GAPDH may play a key role in insulin signal transduction in cardiac muscle.