Researches in enzymes which digest allergic proteins of soybean

在大豆种子萌发和大豆幼苗生长过程中，大豆的3种主要过敏蛋白（β -甘氨酸α亚基、Gly m b28k、Gly m b30k）均显著降低。虽然β -甘氨酸α亚基和Gly m b28k在吸胀后5天消失，但Gly m b30k在吸胀后14天开始下降。结果表明，Gly m bd30k可被另一种降解-甘氨酸α亚基和Gly m bd28k的蛋白酶降解。此外，大豆幼苗制备的溶液在培养过程中，Gly m bd28k含量降低。天门冬氨酸蛋白酶抑制剂的加入干扰了Gly m b28k的体外降低。说明有某种天冬氨酸蛋白酶能降解Gly m b28k。

We revealed that three major allergic proteins (alpha-subunit of beta-conglycinin, Gly m Bd 28K, Gly m Bd 30K) of soybean decrease remarkably under soybean seed germination or soybean seedling growth. Although alpha-subunit of beta-conglycinin and Gly m Bd 28K have disappeared until 5days after imbibition, Gly m Bd 30K started to decrease since 14days after imbibition. It shows that Gly m Bd 30 K is degraded by another protease from that degrade alpha-subunit of beta-conglycinin and Gly m Bd 28K. In addition, Gly m Bd 28K in solution prepared from soybean seedlings decreased when its solution was incubated. Such in vitro decrease of Gly m Bd 28 K was disturbed by addition of aspartic protease inhibitor. It indicates that some kind of aspartic protease degrade Gly m Bd 28K.