Analysis of antisense RNA and development of new diagnostics of gastrointestinal cancer using blood.

本研究以分析反义RNA为目的，通过对大肠癌患者血液中20种反义RNA的检测，发现其在正常人血液中存在明显的变化。这些反义RNA可以清楚地区分健康人和结肠癌患者。我们还鉴定了15种miRNA和17种miRNA，分别在结肠癌患者和胰腺癌患者的血液外泌体中变化为健康人。这些miRNA可以很好的区分健康人和结直肠癌患者，为开发新的低成本的胃肠道癌基因芯片奠定了基础。

The aim of this research is to analyze of antisense RNA and develop of new diagnostics of gastrointestinal cancer using blood.We identified 20 anti-sense RNA that is changing with the healthy people in the blood of patients with colorectal cancer. These antisenseRNA was possible to clearly distinguish the healthy people and colon cancer patients. We also identified 15 miRNA and 17 miRNA, respectively, changing to healthy people in the blood of exosomes in patients with colon cancer and pancreatic cancer patients. These miRNA was possible to distinguish clearly healthy people and patients with colorectal cancer.From these results, we will develop new low cost custom microarray for diagnosis of gastrointestinal cancer.