Cell culture laboratory and animal facility for the cell and animal based projects of the Forschungsgruppe 449

Forschergruppe的几个项目正在处理宿主与病原体的相互作用。为了在Forschergruppe中获得优化的合作和协同效应，Forschergruppe的细胞微生物学实验将在医学微生物学研究所的普通细胞培养设施中进行。例如，上皮细胞(HeLa)、内皮细胞(HUVECs)、巨噬细胞、树突状细胞或成纤维细胞的细胞培养将在这个中央细胞培养实验室按照高度标准化的方案进行。此外，细胞感染实验、原代细胞的准备和细胞库存的准备，包括细胞库存的管理，将在这个中央单元进行。这一设施将确保细胞培养实验的高水平重复性。同样，医学微生物学研究所的一个中心动物设施提供了良好的无特定病原体的动物饲养条件，包括体外培养细胞和一些基因靶向的小鼠品系，这些品系可以被Forschergruppe的各种项目用于体外和体内实验，以研究细菌细胞膜和宿主的相互作用。

Several projects of the Forschergruppe are dealing with host-pathogen interactions. To obtain optimized cooperations and synergistic effects whithin the Forschergruppe, cultivation of mammalian cells for cellular microbiology experiments of the Forschergruppe will be performed in a common cell culture facility at the Instiute for Medical Microbiology. Cell culture of, e.g., epithelial cells (HeLa), endothelial cells (HUVECs), macrophages, dendritic cells or fibroblasts will be performed according to highly standardized protocols in this central cell culture laboratory. Additionally, cell infection experiments, preparation of primary cells and preparing cell stocks including management of the cell stocks will be performed in this central unit. This facility will ensure a high level of reproducibility of cell culture experiments. Likewise a central animal facility at the Institute of Medical Mirobiology provides excellent specific-pathogen free animal housing conditions with IVCs and a number of gene-targeted mouse strains that can be used by the various projects of the Forschergruppe for in vitro and in vivo experiments to study the interactions of bacterial cell envelope and the host.