Mechanism of enhancing vitamin E activity by sesame seed or its lignans

芝麻籽或其木脂素增强维生素E活性的机制在三个实验中进行了研究。在实验1中，我们检测了大鼠肝脏中α-生育酚转移蛋白（alphaTTP）的浓度，这些大鼠喂食对照饮食或含20%芝麻籽的饮食。使用Arai博士（部门）捐赠的免疫抗体，通过免疫抗体法评价浓度。药理学，东京大学）。结果表明，对照大鼠和芝麻种子喂养大鼠之间的alphaTTP浓度没有差异。在实验2中，我们研究了芝麻木脂素（芝麻素和芝麻胺醇）对大鼠由0.5%DHA喂养产生的脂质过氧化物的抑制作用。在喂食DHA的大鼠中，血浆、肝脏和大脑中的脂质过氧化物水平显著增加，血浆和肝脏中的α-生育酚水平下降，但大脑中没有。添加芝麻素和芝麻酚显著抑制脂质过氧化物的产生，并提高血浆、肝脏和大脑中的α-生育酚水平。这些影响几乎是相同的，无论是芝麻素或sesaminol.In实验3中，γ-生育酚的浓度在大鼠的大脑中喂养芝麻种子，其中只含有g-生育酚，相比，大鼠喂养γ-生育酚。在芝麻种子喂养组中发现高γ-生育酚浓度，但在γ-生育酚喂养组中发现脑中γ-生育酚的量可忽略不计。此外，α-生育酚在喂食芝麻籽的大鼠脑中的浓度高于未喂食芝麻籽的对照组。

Mechanisms enhancing vitamin E activity by sesame seed or its lignans were investigated in three experiments.In experiment 1 we examined the concentration of the liver alpha-tocopherol transfer protein (alphaTTP) in the liver of rats fed a control diet or a 20% sesame seed containing diet. The concentration was evaluated by the immuno-antibody method using immuno-antibodies kindly donated by Dr. Arai (Dept. of Pharmacology, Tokyo University). The results showed no difference in the concentrations of alphaTTP between control rats and sesame seed fed rats.In experiment 2 we investigated the suppressing effect of sesame lignans (sesamin and sesaminol) on lipid peroxides produced by 0.5 % DHA feeding in rats. In rats fed DHA, lipid peroxide levels significantly increased in the plasma, liver and brain, and alpha-tocopherol levels decreased in the plasma and liver but not the brain. The addition of sesamin and sesaminol significantly inhibited production of lipidperoxide and raised alpha-tocopherol levels in the plasma, liver and brain. These effects were nearly the same with either sesamin or sesaminol.In experiment 3, gamma-tocopherol concentration in the brain of rats fed sesame seed, which contains only g-tocopherol, was compared to that of rats fed gamma-tocopherol. A high gamma-tocopherol concentration was found in tha sesame seed fed group, but negligible amounts of gamma-tocopherol in brain were found in the gamma-tocopherol-fed group. Further, the concentration of alpha-tocopherol in the brain of rats fed sesame seed with alpha-tocopherol was higher than that of control group without sesame seed.