Molecular mechanisms of the membrane disorder in the senile dementia due to free radical

脑血管型老年期痴呆是由自由基相关的过氧化脂质引起的脑缺血损伤所致。首先，我们研究了过氧化氢胆固醇的鉴定和定量方法。接下来，我们在尸检中收集了痴呆症患者和健康受试者的脑组织。然后将其分为额叶皮质和白质、顶颞叶皮质和白质、枕叶皮质和白质六个区域。将每个组织匀浆，提取脂肪。采用高效液-质联用技术鉴定了7-羟基胆固醇、7-酮类胆固醇和5，6-环氧类胆固醇。此外，我们还用高效液相色谱-化学发光法测定了7α和7β羟基过氧化胆固醇的含量。我们发现老年性痴呆症患者大脑中有7-羟基过氧胆固醇的积聚。在脑区的顶颞部白质有明显的积聚。

Senile dementia of cerebrovascular type is caused by ischemic injury of brain due to free radical-related lipid peroxide. First of all we investigated the methods for identification and quantification of cholesterol hydroperoxide. Next, we collected brain tissue of dementia and healthy subjects at autopsy. Then we divided into six regions, frontal cortex and white matter, parietotemporal cortex and white matter, and occipital cortex and white matter. Each tissue was homogenized and lipid was extracted. We identified 7-hydroperoxycholestero l 7-hydroxycholesterol, 7-ketocholesterol, and 5,6-epoxycholesterol by high performance liquid chromatography-mass spectrometory. In addtion we quantified 7 alpha -and 7- beta hydroperoxycholesterol by high performance liquid chromatography with chemiluminescent detection. We found an accumulation of 7-hydroperoxycholesterol in the brain of senile dementia. The accumulation was evident in parietotemporal white matter among the brain regions.