Simultaneous detection of miltiple STR loci

建立了VWA、F13AO1和FES三个STR基因座的荧光PCR扩增方法。等位基因频率分别为：VWA（n = 114）14：0.110; 15：0.035; 16：0.215; 17：0.250; 18：0.250; 19：0.118; 20：0.022; FES（n = 103）9：0.015; 10：0.087; 11：0.022。0.364;十二日：0.320;十三日：0.209; 14日：0.005，F13A01（n = 114）2.3：0.356; 4：0.081; 5：0.021; 6：0.542。X、Y染色体STR基因座在法医学中的应用近年来得到证实。一个四重PCR，使用2个X-和2个Y-STR（STRX1/HPRTB和DYS390/DYS393），和2个双链PCR，每个使用X-和一个STR（ARA/DYS390和ARA/DYS393），并通过使用自动DNA测序仪进行PCR产物的检测。这种方法使我们不仅可以确定样本供体的性别，还可以确定样本的X和/或y-STR基因型。男性生物样本在quayadruplex-PCR中产生4个扩增产物，在SDS-PCR中产生2个扩增片段，而女性生物样本在quayadruplex-PCR中仅产生2个X-STR扩增片段，在SDS-PCR中也产生1个X-STR扩增片段。因此，我们的研究提供了有用的信息，在法医实践中的许多活动，如身份检验，亲子鉴定，特别是缺乏的情况下，汇编popilation数据，和性别确定的生物样品从一个单一的PCR。

A protoco1 for simultanecus amplification of three STRs loci (VWA, F13AO1 and FES) detected by a fluorescence based technology was performed. Allele frequencies were as follws : VWA(n=114) 14 : 0.110 ; 15 : 0.035 ; 16 : 0.215 ; 17 : 0.250 ; 18 : 0.250 ; 19 : 0.118 ; 20 : 0.022, FES(n=103) 9 : 0.015 ; 10 : 0.087 ; 11 : 0.364 ; 12 : 0.320 ; 13 : 0.209 ; 14 : 0.005, F13A01 (n=114) 2.3 : 0.356 ; 4 : 0.081 ; 5 : 0.021 ; 6 : 0.542. Hardy-Weinberg equilibrium was observed for three loci.The forensic usefulness of X and Y chromosomal STR loci has recently been demonstrated. One quadruplex-PCR, using 2 X- and 2 Y-STRs (STRX1/HPRTB and DYS39O/DYS393), and 2 duplex-PCRs, each using and X- and a STR (ARA/DYS39O and ARA/DYS393), and detection of PCR prcducts was performed by using an automated DNA sequencer. This approach allows us to determine not only the sex of the donor of a sample, but also the X- and/or y-STR genotypes of the sample. A male biological specimen yields 4 amplified products in quayadruplex-PCR and 2 amplified fragments in duplex-PCRs, whereas a female biological specimen yields only 2 amplified fragments of X-STR in quadruplex-PCR and one fragment, also of X-STR, in duplex-PCRs. Our study thus provides useful information for many activities in forensic practice, such as identity testing, paternity testing, especially of deficiency cases, compilation of popilation data, and sex determination of a biological sample from a single PCR.