Extending NARMS surveillance of antibiotic resistance in retail foods in California

项目摘要/摘要 抗菌素耐药性(AMR)是影响食品安全和公众健康的重要问题。这个 年国家抗生素耐药性监测系统(NARMS)零售食品监测 南加州始于2018年。该项目将在最大程度上继续监测 人口稠密的洛杉矶，并将监控范围扩大到加利福尼亚州的农村地区。这个 项目改善食品安全和保护公众健康的目标将通过以下方式实现 承担下列具体目标和活动。首先，我们将继续NARMS零售 在洛杉矶市进行食品监测，并将监测范围扩大到两个农村地区 (阿玛多和卡拉维拉斯)在加利福尼亚州。第二，我们将监测AMR在不同地区的趋势 零售肉类和海鲜样本中的病原体。我们将收集零售肉类(鸡肉，火鸡， (猪肉、牛肉)和海鲜(虾、三文鱼、罗非鱼)样本每月随机抽取 杂货店。我们会化验肉类样本中的沙门氏菌、弯曲杆菌(只限家禽)、大肠杆菌。 以及肠球菌、气单胞菌、弧菌和其他乳糖的肠球菌和海产品样本 根据NARMS的零售食品规程，阳性细菌。细菌分离物将被送往 FDA每月进行抗生素敏感性试验(AST)和全基因组测序(WGS) (肉类分离的WGS将由加州大学戴维斯分校完成)。第三，我们将进行流行病学 零售食品中AMR流行情况的研究。我们将收集商店信息和元数据，以 每个零售肉类和海鲜样本和确定之间的关键环节的流行 细菌及其AMR(如单一或多重耐药)和物种、来源、类型和 零售食品的形式，抗生素使用的声明，以及季节等。第四，根据这一结果 在这个项目中，我们将开发非专业出版物、外展材料、摘要和海报，以及同行-- 回顾了出版物。我们将向农业和水产养殖业社区传播研究结果， 兽医，以及通过时事通讯、推广工作坊、在会议和 会议和同行评议的期刊。五是参加NARMS电话会议 和工作组。 通过实现这些具体目标和活动，该项目将体现以下趋势 加利福尼亚州零售食品中的AMR。该项目将加强NARMS零售食品 监测和提高公众对零售食品中AMR的认识。该项目还将 加强联邦和州机构以及学术界在研究、检测、 监测和调查零售食品中与AMR有关的食源性疫情。 1

Project Summary/Abstract Antimicrobial resistance (AMR) is an important issue for food safety and public health. The National Antimicrobial Resistance Monitoring System (NARMS) Retail Food Surveillance in southern California started in 2018. This project will continue the surveillance in the most populated city of Los Angeles and expand the surveillance to rural areas in California. The project’s goal of improving food safety and protecting public health will be achieved by undertaking the following specific aims and activities. First, we will continue the NARMS retail food surveillance in the city of Los Angeles and expand the surveillance to two rural areas (Amador and Calaveras) in California. Second, we will monitor trends of AMR in different pathogens in retail meat and seafood specimens. We will collect retail meat (chicken, turkey, pork, beef) and seafood (shrimp, salmon, tilapia) specimens monthly from randomly selected grocery stores. We will test meat samples for Salmonella, Campylobacter (poultry only), E. coli and Enterococcus and seafood samples for Enterococcus, Aeromonas, Vibrio and other lactose positive bacteria according to NARMS’ Retail Food Protocols. Bacterial isolates will be sent to FDA monthly for antimicrobial susceptibility test (AST) and whole genome sequencing (WGS) (WGS of meat isolates will be done by UC Davis). Third, we will conduct epidemiological studies on prevalence of AMR in retail food. We will collect store information and metadata for each retail meat and seafood specimen and determine key links between the prevalence of bacteria and their AMR (e.g. single or multiple drug resistance) and species, origins, types, and forms of retail food, claims of antibiotics use, and seasons etc. Fourth, based on results of this project, we will develop lay publications, outreach materials, abstracts and posters, and peer- reviewed publications. We will disseminate findings to agriculture and aquaculture communities, veterinarians, and the public via newsletters, extension workshops, presentations in meetings and conferences, and peer-reviewed journals. Fifth, we will participate in NARMS conference calls and working groups. By accomplishing these specific aims and activities, the project will characterize the trends of AMR in retail foods in the state of California. The project will enhance NARMS Retail Food Surveillance and increase public awareness of AMR in retail foods. The project will also strengthen collaborations among federal and state agencies and academia on research, detection, surveillance, and investigation of foodborne outbreaks associated with AMR in retail food. 1