KOMP2-Phase 2 Production and Phenotyping by the DTCC Consortium

完成KOMP2项目目标因新冠肺炎疫情在DTCC中断 UM1OD023221行政副刊 2021年8月1日-2022年7月31日 摘要 新发严重急性呼吸综合征冠状病毒2型暴发疫情 病(新冠肺炎)在2020年初，也就是KOMP2项目的第10个年头，导致 DTCC联盟成员在几个月内进行的除必要的面对面研究活动外的所有活动 在加州大学戴维斯分校和表观基因组学中心(TCP)。大流行期间的限制措施 减少小鼠繁殖和表型鉴定活动，以最大限度地保持身体距离和 以减少病毒传播和传播的方式运作。因此，在加州大学戴维斯分校工作和 在一年中的大部分时间里，TCP都严重缩减，仅限于保持低于预期的 队列繁育水平和小鼠表型减少。因此，DTCC 财团缺少表型线的总数。因此，DTCC财团 将无法用可用资金实现其表型目标，因此无法满足项目要求 预期在2021年7月31日本项目年度结束前完成。与之相称的 加州大学戴维斯分校和技术合作伙伴的工作活动中断，科学和预算规划进程 同样在NIH停滞不前，这阻止了它发布与KOMP相关的资金 2020年的商机公告。因此，DTCC-KOMP2财团被拒绝 有机会申请竞争性续签。 这一行政补充请求的目的是完成DTCC的生成 财团有义务使用CRISPR/Cas9技术交付1000个突变小鼠品系， 进行表型分析，进行质量控制评估，超低温保存种质， 并使鼠标和数据随时可供研究界使用。尽管……的目标 生产和冷冻保存1000个突变小鼠系已经达到了表型目标 不会的。随着为应对大流行而实施的工作限制解除，预计 成体和胚胎表型管道将在本日历年晚些时候完全恢复。在……里面 在这种情况下，对项目完成的预测进行了调整，以便能够完成 在截至2022年7月31日的一年延长活动期间，所有KOMP2-阶段2项目目标。 因此，DTCC财团请求为一年的资金延期提供行政支持 KOMP2-阶段2赠款，用于与父母赠款的具体目标有关的活动 活动，包括技术人员、表型鉴定用品、每日小鼠和 维护成本、信息支持和项目协调。这些活动将使 26例早期成人纯合子和46例早期成人完成队列生产和表型分析 成虫杂合系，31个胚胎致死/亚存活系，8个晚成体纯合系。 此外，另外28个胚胎亚系将被复活，以产生胚胎 因为新冠肺炎而延迟和损坏的影像。所有这些活动的完成将 使DTCC联合体能够履行其对KOMP2-Phase2项目的所有义务。

Completion of KOMP2 Project Goals Interrupted by the COVID-19 Pandemic at the DTCC UM1OD023221 Administrative Supplement August 1, 2021 – July 31, 2022 ABSTRACT The outbreak of emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) disease (COVID-19) early in 2020, the 10th year of the KOMP2 Project, caused a suspension of all but essential in-person research activities for several months by DTCC Consortium members at UC Davis and The Centre for Phenogenomics (TCP). Restrictions during the pandemic curtailed mouse breeding and phenotyping activity in order to maximize physical distancing and operate in a way that mitigates viral transmission and spread. As a result, work at UC Davis and TCP was severely curtailed for most of the year, limited to maintaining a lower-than-expected level of breeding of cohorts and reduced phenotyping of mice. Consequently, the DTCC Consortium is short of the total number of lines phenotyped. Therefore, the DTCC Consortium will be unable to fulfill its phenotyping goals with available funds and thus will not meet project expectations by the end of the current project year on July 31, 2021. Commensurate with the disruption in work activities at UC Davis and TCP, scientific and budgetary planning processes were similarly stalled at NIH which prevented it from publishing a KOMP-related funding opportunity announcement in 2020. As a result, the DTCC-KOMP2 Consortium was denied the opportunity to apply for a competitive renewal. The aim of this administrative supplement request is to finish the generation of the DTCC Consortium obligation to deliver 1000 mutant mouse lines using CRISPR/Cas9 technology, perform phenotyping assays, conduct quality control assessments, cryopreserve germplasm, and make mice and data readily available to the research community. Although the goal of producing and cryopreserving 1000 mutant mouse lines has already met, the phenotyping goals will not be. As work restrictions imposed in response to the pandemic lift, it is expected that adult and embryo phenotyping pipelines will be fully restored later during this calendar year. In this context, projections for completion of the project have been adjusted to enable completion of all KOMP2-Phase2 project goals during a 1-year extension of activities ending July 31, 2022. Therefore, the DTCC Consortium requests administrative support for a 1-year funded extension of the KOMP2-Phase2 grant for activities related to the Specific Aims of the Parent Grant activities, including technical personnel, phenotyping supplies, mouse per diem and maintenance costs, informatics support, and project coordination. These activities will enable completion of cohort production and phenotyping of 26 early adult homozygous and 46 early adult heterozygous lines, 31 embryo lethal/subviable lines, and 8 late adult homozygous lines. In addition, another 28 embryo subviable lines will be reanimated to generate embryos for imaging that were delayed and spoiled due to COVID-19. Completion of all these activities will enable the DTCC Consortium to fulfill all of its obligations to the KOMP2-Phase2 project.