In Vitro Models and Cell Culture Core

项目摘要/摘要-体外模型细胞培养核心(核心B) 建立体外模型和细胞培养核心，为体外培养提供良好分化的上皮细胞模型。 囊性纤维化的病理生理学研究以及分子和基因治疗实验。最新消息 体外模型和细胞培养的核心任务是促进CF研究，以影响CF患者的健康。 核心通过向调查人员提供相关模型和技术专长来实现这一使命， 新的模式和方法，培训调查人员，并吸引新的调查人员参与CF研究和援助 他们的发展。体外模型和细胞培养核心的目标是收集、加工和生物库 人、猪和雪貂的组织和呼吸道上皮细胞。Core还维持着人类非CFs的生物库 和CF上皮，包括罕见突变的CF上皮。核心还将描述模型和本机 上皮细胞与其他核心和研究人员合作，并将进行生物电分析， 形态评估、基因表达谱和流式细胞术。最后，核心将教授 爱荷华大学和其他机构的研究人员如何成功实施这些方法 体外模型系统。

PROJECT SUMMARY/ABSTRACT - In Vitro Models Cell Culture Core (CORE B ) The In Vitro Models and Cell Culture Core was established to provide well-differentiated epithelial models for studies on the pathophysiology of cystic fibrosis (CF) and for molecular and gene therapy experiments. The In Vitro Models and Cell Culture Core mission is to advance CF research to impact the health of people with CF. The Core achieves that mission by providing relevant models and technical expertise to investigators, developing new models and approaches, training investigators, and attracting new investigators to CF research and aiding their development. The goals of the In Vitro Models and Cell Culture Core are to collect, process and biobank tissue and airway epithelia from human, pig, and ferret. The Core also maintains a biobank of human non-CF and CF epithelia, including CF epithelia with rare mutations. The Core will also characterize models and native epithelia in collaboration with other Cores and investigators and will perform bioelectric analyses, morphological evaluation, gene expression profiles, and flow cytometry. Finally, the Core will teach investigators at the University of Iowa and other institutions how to successfully implement the methods for in vitro model systems.