Research Project for NSRRC Renewal Year 21-25

应用研究（Applied Research） 本申请的总体目标是继续和扩大国家猪资源的运作， 研究中心（NSRRC）。在过去的资助期间，NSRRC开发了一种非侵入性方法， 通过超快速冷却冷冻保存胚胎，改进体细胞发育方法 核移植胚胎，开发了一种通用的性别决定测定法，并将GHR敲除 为了减小家猪的尺寸同时保持其上级繁殖性能， 并开发了最先进的疾病监测和监测分析。的具体目标 研究核心是进行创新研究，这将有利于NSRRC和生物医学研究 社区应用研究项目旨在提高a）猪生殖器官的冷冻保存 细胞，和B）开发用于生产基因工程猪的改进方法。

Abstract (Applied Research) The overall goal of this application is to continue and expand operation of the National Swine Resource and Research Center (NSRRC). During the past funding period, the NSRRC developed a non-invasive method of cryopreservation of embryos via ultra-fast cooling, improved methods for the development of somatic cell nuclear transfer embryos, developed a universal sex determining assay, and incorporated a GHR knockout in an attempt to reduce the size of domestic pigs while retaining their superior reproductive performance, and developed state-of-the-art disease monitoring and surveillance assays. The Specific Aim of the Research Core is to perform innovative research that will benefit the NSRRC and the biomedical research community. Applied research projects are aimed at improving a) the cryopreservation of pig reproductive cells, and b) the development of improved methods for the production of genetically-engineered pigs.