A&R

改建和装修摘要 UMaine的斑马鱼设施对COBRE上的大多数研究项目负责人（RPL）至关重要。 总体而言，12 UMaine教师，他们的技术人员，本科生和研究生使用斑马鱼设施 他们的研究每年。这些教师代表了缅因大学的许多不同单位，包括 分子和生物医学科学系，生物学和生态学学院， 海洋科学。斑马鱼已经被缅因大学的教职员工用来研究许多不同的疾病过程， 包括流感、肌肉萎缩症和念珠菌真菌感染。斑马鱼设施也是相关的 缅因大学的电气和计算机工程等学科的教师，他们正在开发设备， 技术，以解决预防和治疗人类疾病的过程。 缅因州大学目前的斑马鱼设施，位于同一建筑物作为缅因州大学的RPL和拟议的 显微镜和图像分析核心，通常以98-100%的容量运行。虽然一个优秀的设施 这是由缅因州大学资助的93%的基础;有限的能力阻碍了所有使用 斑马鱼模型。该设施是3个COBRE RPL维持和开发斑马鱼的重要资源 用于其拟议研究项目的线路。作为我们COBRE的一部分，我们建议扩大UMaine的 斑马鱼要做到以下几点：增加育苗空间，便于新品系快速生长， 产卵空间，以加快实验步伐，并几乎两倍的能力，我们目前的系统。我们将 通过完成两个具体目标来做到这一点。具体目标1：将教学实验室转变为附加实验室 斑马鱼设施。这个改造项目将把一个1,242平方英尺的教学实验室改造成希契纳大厅， 穿过大厅，进入另一个斑马鱼实验室。将此实验室添加到 整个斑马鱼设施将使斑马鱼住房的可用空间增加一倍以上。翻修将 包括拆除现有空间，倾斜地板，安装排水沟，HVAC，电气 工作，并安装热泵。具体目标2：购买集中式水循环系统， 坦克架和坦克。在COBRE资助的范围内，并承诺从2011年12月15日起提供154，000美元， 缅因大学研究副校长办公室，我们将为决赛安装集中式基础设施 坦克机架的数量，立即购买和安装机架和坦克，将立即 将我们的产能提高了91%购买交叉货架将使每日养殖能力从25个增加到 每天175个坦克。这种增加的能力对于COBRE研究项目领导者（RPL）来说是必要的， 使用斑马鱼模型，将为缅因大学的所有斑马鱼研究人员带来变革。

Alteration and Renovation Abstract UMaine’s Zebrafish Facility is critical to the majority of the Research Project Leaders (RPLs) on this COBRE. Overall, 12 UMaine faculty, their technicians, undergraduate, and graduate students use the Zebrafish Facility for their research on an annual basis. These faculty represent a number of different units at UMaine including the Department of Molecular and Biomedical Sciences, the School of Biology and Ecology, and the School of Marine Sciences. Zebrafish have been used by UMaine faculty studying many different disease processes, including influenza, muscular dystrophy, and Candida fungal infections. The Zebrafish Facility is also relevant to UMaine faculty in disciplines such as electrical and computer engineering, who are developing devices and technologies to address the prevention and treatment of human disease processes. UMaine’s current zebrafish facility, located in the same building as the UMaine RPLs and the proposed Microscopy and Image Analysis Core, typically operates at 98-100% capacity. Although an excellent facility that is 93% base-funded by the University of Maine; the limited capacity hinders all investigators who use the zebrafish model. This facility is an essential resource for 3 COBRE RPLs to maintain and develop zebrafish lines used for their proposed research projects. As part of our COBRE we are proposing to expand UMaine’s Zebrafish to accomplish the following: increase nursery space to facilitate rapid growth of new lines, increase spawning space to increase experimental pace, and nearly double the capacity of our current system. We will do this by completing two specific aims. Specific Aim 1: Convert a Teaching Laboratory into an Additional Zebrafish Facility. This renovation project will convert a 1,242 ft2 teaching laboratory in Hitchner Hall, that is across the hall from the current facility, into an additional Zebrafish Laboratory. The addition of this lab to the overall zebrafish facility will more than double the available space for zebrafish housing. Renovations will include demolition of the current space, sloping of the floor, installation of a trench drain, HVAC, electrical work, and installation of a heat pump. Specific Aim 2: Purchase a centralized water circulation system, tank racks, and tanks. Within the scope of COBRE funding, as well as with a commitment of $154,000 from the Office of the Vice President for Research at UMaine, we will install the centralized infrastructure for the final number of tank racks, the immediate purchase and installation of racks and tanks that will immediately increase our capacity by 91%. Purchase of crossing shelves will increase daily breeding capacity from 25 to 175 tanks per day. This increased capacity is necessary for the COBRE research project leaders (RPLs) that use the zebrafish model and will be transformative for all of the zebrafish researchers at UMaine.