RAPID CYTOMETRIC ASSAY FOR LYMPHOCYTE ACTIVATION

淋巴细胞活化的快速细胞测定法有很多潜力 临床应用，包括细胞免疫功能障碍的评估 在艾滋病毒感染和癌症中，评估自身免疫的严重程度 移植受者的疾病过程、组织分型和监测 以寻找移植排斥的早期迹象。着眼于发展…… 可重复的、标准化的方法，可在当前 新一代临床流式细胞仪、多参数流式细胞仪将 用于分析和关联RNA含量、激活 人的抗原表达和膜示踪染料荧光 外周T淋巴细胞在激活后24小时内 在一项多克隆有丝分裂原、同种异体抗原或错配细胞的试验中 几种细胞活化指标的有效性比较 用氚胸苷掺入。很可能这些研究 将验证基于检测到的RNA含量增加的分析 派罗宁Y和/或CD98激活抗原的表达。 拟议的商业应用：淋巴细胞激活的快速测试 在疾病的诊断和监测中具有广泛的应用潜力 免疫系统紊乱。

Rapid cytometric assays for lymphocyte activation have many potential clinical applications, including evaluation of cellular immune dysfunction in HIV infection and cancer, assessment of the severity of autoimmune disease processes, tissue typing, and monitoring of transplant recipients for early signs of graft rejection. With a view toward development of reproducible, standardized methods which can be implemented on the current generation of clinical flow cytometers, multiparameter flow cytometry will be used to analyze and correlate changes in RNA content, activation antigen expression, and membrane tracking dye fluorescence in human peripheral T-lymphocytes during the first 24 hours following activation by polyclonal mitogens, alloantigen, or mismatched cells, in a trial of the efficacy of these cytometric indicators of cell activation in comparison with tritiated thymidine incorporation. It is likely that these studies will validate an assay based on detection of RNA content increases using pyronin Y and/or expression of the CD98 activation antigen. PROPOSED COMMERCIAL APPLICATION: Rapid tests for lymphocyte activation have a wide potential range of application in diagnosis and monitoring of immune system disorders.