1H NMR MEASUREMENTS OF BRAIN GABA CONCENTRATION

GABA能系统在AS的发病机制中可能起重要作用 以及在癫痫和其他脑部的药物控制中 疾病。GABA的浓度和合成速率可以反映 GABA能系统的活动。拟议研究的目标是 建立定量的单体积和多体积核磁共振氢谱方法 脑内γ-氨基丁酸(GABA)浓度和速率的测定 在人脑中的合成。这些方法将被应用于研究 人类受试者要确定测量的精度， 共振分配的精确度，并测量松弛 GABA绝对定量所需的性质。一个动物模型会 通过与常规核磁共振方法的比较，验证了核磁共振方法的有效性 化学化验。~1H核磁共振测定GABA方法的研究进展 将为研究人类GABA能提供一个重要的新工具 系统。

The GABAergic system may play a significant role in the pathogenesis as well as in the pharmacological control of epilepsy and other brain diseases. Concentrations and rates of synthesis of GABA may reflect the activity of the GABAergic system. The goal of the proposed research is to develop quantitative single volume and multivolume 1H NMR methods for measuring brain gamma amino butyric acid (GABA) concentration and rates of synthesis in human brain. The methods will be applied in studies of human subjects to determine the precision of the measurement, the accuracy of the resonance assignment, and to measure the relaxation properties needed for GABA absolute quantitation. An animal model will be used to validate the NMR method by comparison with conventional chemical assay. The development of 1H NMR methods for measuring GABA will provide an important new tool for studying the human GABAergic system.