IN VITRO MEASUREMENT OF BINDING OF SELF AND ANTIGENIC PEPTIDES TO MHC MOLECULES

这项工作的目的是了解的细节， 自身和外源抗原肽与MHC I类的相互作用 分子通过详细的动力学和平衡结合方法。 在 除了让我们了解潜在的生物化学规则 控制蛋白质/肽相互作用，这些研究提供了具体的 关于特定MHC/肽相互作用的信息， 启动免疫反应。 在过去的一年里，我们进一步改进了定量分析， 研究了许多蛋白质/肽相互作用，包括 抗体/肽相互作用。 在A上理解这些过程 生物化学和生物物理水平不仅为 了解结合反应，但也提供了一个机会， 可能用于免疫的肽类似物的合理设计 和自身免疫性疾病的干预。

The objective of this work has been to understand the details of the interaction of self and foreign antigenic peptides with the MHC class I molecule by detailed kinetic and equilibrium binding methods. In addition to allowing us to understand the underlying biochemical rules that govern protein/peptide interactions, these studies provide specific information on particular MHC/peptide interactions which govern the initiation of the immune response. In the past year we have further improved upon quantitative assays, examined a number of protein/peptide interactions, including antibody/peptide interactions. Understanding these processes on a biochemical and biophysical level provides a basis not only for understanding the binding reactions, but also offers an opportunity for rational design of peptide analogs that might be useful in immunization and intervention in autoimmune disease.