ANTIGEN SCANNING ON COMBINATORIAL ARRAYS

描述：(改编自申请人的摘要)组合 合成一系列新化合物的技术正在迅速发展 开发，导致治疗和诊断的新候选者 治疗。相比之下，开发利用这些资源的技术 组合库一直是相对静态的。在每种方法中， 识别步骤依赖于对感兴趣的抗原的强吸附 图书馆的一名成员。这一认识步骤可能会忽略温和的 相互作用或细微的差异，可以为 进一步筛查。此外，这一步骤还限制了MIXED 筛选方案中的文库。 他们提议制定一项新的承认步骤，以绕过 克服困难。该方法适用于组合库 在光滑平坦的表面上。该方法涉及到直接测量 利用原子力使抗原与每个文库成员相互作用 显微镜。如果成功，建议的方法将提供一个 定量衡量每个图书馆成员与一个 感兴趣的抗原。这将是一种新的技术来表征 抗原。该方法还将加强混合图书馆在 筛查方案。这可能会带来高效的筛查 需要较少数量的库成员的协议。 建议的商业应用：不可用

DESCRIPTION: (Adapted from the applicant's abstract) Combinatorial techniques to synthesize a host of new compounds are being rapidly developed, leading to new candidates for therapeutic and diagnostic treatments. In contrast, the development of techniques to exploit these combinatorial libraries has been relatively static. In every method the recognition step relies on strong adsorption of the antigen of interest to one member of the library. This recognition step could overlook moderate interactions or subtle differences that could provide important clues for further screening. In addition, this step limits the use of mixed libraries in screening protocols. They propose to develop a new recognition step that will circumvent the above difficulties. The method is applicable to combinatorial libraries on smooth, flat surfaces. The method involves the direct measurement of the interaction of the antigen with each library member using atomic force microscopy. If successful, the proposed method would provide a quantitative measure of the interaction of each library member with an antigen of interest. This would be a new technique to characterize antigens. The method would also enhance the use of mixed libraries in screening protocols. This could result in highly efficient screening protocols that require a lower number of library members. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE