PATHOGEN FREE RHESUS MONKEY BREEDING AND RESEARCH

猿猴逆转录病毒--猴T淋巴细胞嗜性病毒I型 免疫缺陷病毒和D型逆转录病毒是重要的动物模型 对人类免疫缺陷的研究也构成了对 圈养家养繁殖群体的健康状况。猴疱疹病毒是一种 已证实对动物护理人员和实验室人员有危险。新的 英格兰地区灵长类动物研究中心(NERPRC)成功 建立了无特定病原体(SPF)的恒河猴繁育群体 提供未感染的实验对象，改善群体健康。 我们建议将种群扩大到250只猴子，以支持这些 关键目标。繁殖群体将自给自足并提供 每年为35只SPF恒河猴提供持续的和联邦资助的 NERPRC的研究项目。未受感染的动物将被确认 使用经过验证的血清学和病毒分离技术。SPF动物将 被集体安置在一个专门的现代混凝土砌块建筑中。防晒系数 将通过定期的血清检测来确保菌落的状态 监视系统。繁育群体的遗传多样性将是 通过跟踪单个动物的谱系来最大限度地避免近亲繁殖 并通过分析定期测定平均杂合度 血液蛋白多态。行为管理和学习将确保 SPF繁殖者具有正常的社会和母性特征。

The simian retroviruses simian T-lymphotropic virus I, simian immunodeficiency virus, and type D retrovirus are important animal models for the study of human immunodeficiency and also pose a threat to the health of captive domestic breeding colonies. Herpesvirus simiae is a proven hazard to animal care workers and laboratory personnel. New England Regional Primate Research Center (NERPRC) has successfully established a specific-pathogen-free (SPF) rhesus monkey breeding colony to provide uninfected experimental subjects and to improve colony health. We propose to expand the colony to 250 monkeys in support of these crucial goals. The breeding colony will be self-sustaining and provide 35 SPF rhesus monkeys annually for the ongoing and Federally funded research programs at the NERPRC. Uninfected animals will be identified using proven serologic and virus isolation techniques. SPF animals will be group housed in a dedicated modern concrete block building. The SPF status of the colony will be ensured through periodic serologic surveillance. The genetic diversity of the breeding colony will be maximized by tracking of individual animal pedigrees to avoid inbreeding and by periodic determination of average heterozygosity through analysis blood protein polymorphism. Behavioral management and study will ensure that SPF breeders have normal social and maternal characteristics.