GENETICS OF T-LYMPHOCYTE ANTIGEN RECEPTORS

我们计划使用免疫细胞化学的方法研究T淋巴细胞抗原识别的遗传学。 T细胞杂交瘤、抗受体抗体和受体的DNA探针 基因. 我们的一般方法是检查V基因的杂交瘤， 已知特异性和相关特异性，以便将 具有Ag/MHC识别的受体结构。 具体项目包括绘制6号染色体上的β基因座， 检测杂交瘤中识别常规抗原的V基因 在一些实施方案中，抗体可以是抗原（卵清蛋白）或同种异体抗原（I-Ab），或自身抗原（Dd）。 我们将计划 为了克隆基因， 基因座 最后，我们建议检查受体的表达， 胸腺发育 最终，我们希望更好地了解Ag/MHC 识别是在基因组中编码的。

We plan to study the genetics of antigen recognition in T lymphocytes using T cell hybridomas, anti-receptor antibodies and DNA probes for receptor genes. Our general approach is to examine the V genes in hybridomas of known specificity and those of related specificity in order to correlate receptor structure with Ag/MHC recognition. Specific projects include mapping the beta locus on chromosome 6, examination of V genes in hybridomas which recognize a conventional antigen (ovalbumin) or an alloantigen (I-Ab), or a self-antigen (Dd). We will plan to sequence an alpha chain polypeptide in order to clone genes in this locus. Finally, we propose to examine receptor expression in the developing thymus. Ultimately, we hope to better understand how Ag/MHC recognition is encoded in the genome.