MURINE LYME BORRELIOSIS

莱姆病是一种最近被描述的螺旋体疾病，这种疾病正在增加。 在美国和欧洲的许多地区流行。很少 了解易感性、潜伏期、免疫学等基本问题， 病理学，以及发病机制。我们对这些问题的无知导致了 对最佳诊断和治疗的不确定性。可靠的动物模型 是必要的。 这项建议的目标是研究一种小鼠感染模型， 莱姆病病原伯氏疏螺旋体及其应用 获取管理信息的模型，两个主要决定因素 感染的是伯氏杆菌菌株和宿主的遗传背景。在……里面 本项目研究了一种易感小鼠品系的感染过程。 伯格多费氏菌将通过调查剂量的影响来研究 有机体、注射途径和文化祭祀日 阳性和器官中细菌和炎症的存在。蜂窝 并将评估感染小鼠的体液免疫反应。 在模型的这一初始表征中获得的信息然后可以 用来探索两个重要的问题：基因决定的 不同品系小鼠的敏感性和传染性的变异性 不同种类的有机体。 从这项工作中获得的信息将提高我们对 莱姆疏螺旋体病通过获取有关事件顺序的信息 最初的感染，感染的器官，随着螺旋体的变化 留在体内，并在感染后诱发病理变化。这些 调查也将有助于我们了解B. Burgdorferi能够建立潜伏期并躲避，或者至少是提出条件 有了宿主的免疫反应。

Lyme disease is a recently described spirochetal illness that is increasing in prevalence in many areas of the United States and Europe. Very little is known about basic issues such as susceptibility, latency, immunology, pathology, and pathogenesis. Our ignorance about these questions leads to uncertainty about optimal diagnosis and treatment. A reliable animal model is needed. The goal os this proposal is to investigate a mouse model of infection by Borrelia burgdorferi, the causative organism of Lyme disease, and to use the model to gain information on management, two major determinants of infection are B. burgdorferi strain and genetic background of the host. In this project the course of infection of a susceptible mouse strain by B. burgdorferi will be studied by investigating the influence of dose of organism, the route of injection, and day of sacrifice on culture positively and presence of bacteria and inflammation in organs. Cellular and humoral immune responses in infected mice will be assessed. Information gained in this initial characterization of the model can then be used to explore two important issues: genetically determined susceptibility in various mouse strains and variability in infectivity in different strains of the organism. The information gained from this work will improve our understanding of Lyme borreliosis by gaining information on the sequence of events after initial infection, the organs infected, changes in the spirochete as it remains in vivo, and pathological changes induced in the infection. These investigations will also aid in our understanding of the means by which B. burgdorferi is able to establish latency and evade, or at least make terms with, the host immune response.