IMMUNOGENETICS OF LY SYSTEMS

在理解细胞程序的构成时， 表面组成，并最终控制，重要的是要知道 编码几种蛋白质的基因的位置 糖蛋白相关 我们报告了19号染色体的Lyt-1位点， 和1号染色体的Ly-5位点，是蛋白质结构的位点， 与组成特定表面表型有关的基因 淋巴细胞分类 Ly-5的一个中心焦点，大多数人都表示 或所有造血细胞，是确定不同的亚型， 表征不同的造血细胞谱系。 它表明，在 这些同种型中至少有两种在蛋白质结构上不同。 一个很严重的问题 现在的问题是，是否以及哪些同种型是独立的、连锁的基因的产物， 其产物共享Ly-5抗原，并且其可能是 转录后修饰，如选择性剪接。 电流 工作重点是验证我们拥有的Ly-5 cDNA探针 克隆，并用于此目的。 缺乏基因表达的变异白血病细胞系的性质和特性 I类基因在许多情况下都是令人感兴趣的，其中之一是 白血病细胞中TL抗原的异常调节。 它示出 其中一种变体表现出整个H-2：Tla的表型丢失 单倍型是真正的基因组变体，其中该单倍型的基因 已经丢失，可能是由于伴随着 其余基因组单倍型的复制。 盟军变种将是 研究的目的是建立任何必要的关系， Tla基因座的保留和恶性肿瘤。 成功地应用于Ly-5的克隆方法使其自身适合于 克隆用于分化抗原系统的其它基因， 实验室已经定义了，特别是Lyt-1，还有Tla， 需要与其它I类基因不同具有充分选择性的探针， 而有关的研究亦在进行中。 （简体中文）

In achieving an understanding of what constitutes a program for cell surface composition, and ultimately its control, it is important to know the location of the genes that code the proteins of the several glycoproteins concerned. We report that the Lyt-1 locus of chromosome 19, and the Ly-5 locus of chromosome 1, are the sites of protein structural genes concerned in composing the surface phenotypes of particular lymphocyte classes. A central focus of interest in Ly-5, expressed by most or all hematopoietic cells, is the determination of different isoforms that characterize different hematopoietic cell lineages. It is shown that at least two of these isoforms differ in protein structure. A vital question now is whether and which isoforms are products of separate, linked genes whose produces share Ly-5 antigen, and which may be the products of post-transcriptional modification such as alternative splicing. Current work centers on the verification of an Ly-5 cDNA probe which we have cloned, and its use for that purpose. The nature and properties of variant leukemia lines that lack expression of class I genes are of interest in many contexts, among which is the relation of aberrant regulation of TL antigens in leukemia cells. It is shown that one such variant, which exhibits phenotypic loss of an entire H-2:Tla haplotype is a true genomic variant in which the genes of that haplotype have been lost, probably by somatic recombination accompanied by duplication of the remaining genomic haplotype. Allied variants will be studied with a view to establishing any necessary relation between retention of either Tla locus and malignancy. The cloning methods successfully applied to Ly-5 lend themselves to the cloning of other genes for differentiation antigen systems which this laboratory has defined, in particular Lyt-1, and also Tla, for which a sufficiently selective probe distinct from other class I genes is wanted, and studies to that effect are in hand. (CS)